Journal: Molecular pharmaceutics

Article Title: In Situ Vaccination with Cowpea vs Tobacco Mosaic Virus against Melanoma

doi: 10.1021/acs.molpharmaceut.8b00316

Figure Lengend Snippet: Antibodies Used for Flow Cytometry

Article Snippet: Flow cytometry analyses were performed on a BD LSR II flow cytometer (BD Biosciences), and data was analyzed using the FlowJo software (Tree Star Inc.). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 dye marker vendor innate panel catalog no. clone/host isotype concentration pacific blue CD4S BioLegend 103126 30-F11 Rat IgG2b, K 0.5 mg/mL FITC CD11b BioLegend 10120S Ml/70 Rat IgG2b, K 0.5 mg/mL PE CD80 BioLegend 104707 16–10A1A Hamster IgG 0.2 mg/mL PE-Cy7 CD86 BioLegend 10S014 GL-1 Rat IgG2a, K 0.2 mg/mL APC I-A/I-E (=MHCII) BioLegend 107613 MS/114.1S.2 Rat IgG2b, K 0.2 mg/mL APC/Cy7 Ly-6G BioLegend 127623 1A8 Rat IgG2a, K 0.2 mg/mL APC CD11c BioLegend 10120S N418 A Hamster 0.2 mg/mL PE F4/80 BioLegend 123109 BM8 Rat IgG2a, K 0.2 mg/mL PE/Cy7 Ly6C BioLegend 128018 HK1.4 Rat IgG2c, K 0.2 mg/mL BV60S NK1.1 BioLegend 108737 PK136 IgG2a, k 0.2 mg/mL dye marker vendor adaptive panel catalog no. clone/host isotype concentration pacific blue CD45 BioLegend 103126 30-F11 Rat IgG2b, K 0.5 mg/mL FITC CD4 BioLegend 100405 GK1.5 Rat IgG2b, K 0.5 mg/mL PE CD44 BioLegend 103007 1M7 Rat IgG2b, K 0.2 mg/mL PE-Cy7 CD62L (L-selectin) BioLegend 104417 MEL-14 Rat IgG2a, K 0.2 mg/mL APC CD8 α BioLegend 100712 S3–6.7 Rat IgG2a, K 0.2 mg/mL APC/Cy7 CD3 ε BioLegend 100330 14S-2C11 A. Hamster IgG 0.2 mg/mL Open in a separate window Antibodies Used for Flow Cytometry

Techniques: Marker, Concentration Assay

Journal: Molecular pharmaceutics

Article Title: In Situ Vaccination with Cowpea vs Tobacco Mosaic Virus against Melanoma

doi: 10.1021/acs.molpharmaceut.8b00316

Figure Lengend Snippet: Antibodies Used for Immunohistochemistry

Article Snippet: Flow cytometry analyses were performed on a BD LSR II flow cytometer (BD Biosciences), and data was analyzed using the FlowJo software (Tree Star Inc.). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 dye marker vendor innate panel catalog no. clone/host isotype concentration pacific blue CD4S BioLegend 103126 30-F11 Rat IgG2b, K 0.5 mg/mL FITC CD11b BioLegend 10120S Ml/70 Rat IgG2b, K 0.5 mg/mL PE CD80 BioLegend 104707 16–10A1A Hamster IgG 0.2 mg/mL PE-Cy7 CD86 BioLegend 10S014 GL-1 Rat IgG2a, K 0.2 mg/mL APC I-A/I-E (=MHCII) BioLegend 107613 MS/114.1S.2 Rat IgG2b, K 0.2 mg/mL APC/Cy7 Ly-6G BioLegend 127623 1A8 Rat IgG2a, K 0.2 mg/mL APC CD11c BioLegend 10120S N418 A Hamster 0.2 mg/mL PE F4/80 BioLegend 123109 BM8 Rat IgG2a, K 0.2 mg/mL PE/Cy7 Ly6C BioLegend 128018 HK1.4 Rat IgG2c, K 0.2 mg/mL BV60S NK1.1 BioLegend 108737 PK136 IgG2a, k 0.2 mg/mL dye marker vendor adaptive panel catalog no. clone/host isotype concentration pacific blue CD45 BioLegend 103126 30-F11 Rat IgG2b, K 0.5 mg/mL FITC CD4 BioLegend 100405 GK1.5 Rat IgG2b, K 0.5 mg/mL PE CD44 BioLegend 103007 1M7 Rat IgG2b, K 0.2 mg/mL PE-Cy7 CD62L (L-selectin) BioLegend 104417 MEL-14 Rat IgG2a, K 0.2 mg/mL APC CD8 α BioLegend 100712 S3–6.7 Rat IgG2a, K 0.2 mg/mL APC/Cy7 CD3 ε BioLegend 100330 14S-2C11 A. Hamster IgG 0.2 mg/mL Open in a separate window Antibodies Used for Flow Cytometry

Techniques: